专科技术
时间：2025-08-28    点击数： 次    字体： 

        一、研究方向与特色
        科室聚焦重大疾病领域，结合自身优势，形成了特色鲜明的研究方向：
        1.神经退行性疾病的机制与早筛研究： 重点围绕阿尔茨海默病等，深入探索其发病机理，致力于开发早期、无创的诊断生物标志物与筛查策略。
        2.新型纳米药物载体研发： 着力于新型铁基纳米材料的构建与应用研究，开发智能型药物递送系统，为肿瘤等疾病的精准治疗提供新方案。
         3.肿瘤免疫微环境与介入靶向治疗探索： 利用介入技术优势，研究局部肿瘤微环境的免疫特征，探索介入治疗与靶向药物、免疫治疗相结合的新策略。
        二、目前可开展的项目：
        1、细胞培养实验：包括细胞传代、细胞增殖及细胞毒性检测、细胞免疫荧光实验。细胞培养可以开展细胞通路、细胞凋亡、蛋白定位等研究，是科学研究中常用的试验方法。
        2、大肠杆菌培养实验：大肠杆菌的培养试验、质粒转化试验。大肠杆菌的培养可以表达某种蛋白，也可以克隆基因，是实验人员对基因研究的常规操作方法。
        3、核酸的提取实验：血液和组织的DNA提取、RNA提取、保存等。核酸是开展SNP、测序、基因上调或者下调研究的关键材料，中心实验室具备核酸提取和保存的条件。
        4、SDS-PAGE实验：SDS聚丙烯酰胺凝胶电泳实验，对样本中表达的蛋白进行分析。现已购买Western Blot免疫电泳成像系统，可完成Western Blot试验。
        5、PCR实验：普通PCR实验、荧光定量PCR实验，可以对样本提取的DNA或者RNA进行扩增分析鉴定。
        6、生化指标检测：可以对血清、血浆和组织样本进行超氧化物歧化酶、过氧化氢酶、过氧化物酶、蛋白含量等生化指标的检测。
        7、ELISA实验：可以对血清或者血浆样本进行各种ELISA测定试验。中心实验室可以开展样本中细胞因子的测定，抗体的测定等。

Ⅰ. Research Directions and Features
The department focuses on major disease areas and has formed distinctive research directions based on its own advantages:
1.Research on the Mechanisms and Early Screening of Neurodegenerative Diseases: Focusing on Alzheimer's disease and others, in-depth exploration of its pathogenesis, committed to developing early, non-invasive diagnostic biomarkers and screening strategies.
2.Development of Novel Nano Drug Carriers: Dedicating efforts to the construction and application research of novel iron-based nanomaterials, developing intelligent drug delivery systems to provide new solutions for the precise treatment of diseases such as tumors.
3.Exploration of Tumor Immune Microenvironment and Interventional Targeted Therapy: Leveraging the advantages of interventional technology to study the immune characteristics of the local tumor microenvironment and explore new strategies combining interventional therapy with targeted drugs and immunotherapy.

Ⅱ. Currently Available Projects:
1.Cell Culture Experiments: Including cell passaging, cell proliferation and cytotoxicity detection, and cell immunofluorescence experiments. Cell culture can be used for research on cell pathways, apoptosis, protein localization, etc., and is a commonly used experimental method in scientific research.
2.E. coli Culture Experiments: E. coli culture experiments, plasmid transformation experiments. E. coli culture can be used to express specific proteins or clone genes, and is a routine operation method for genetic research by experimenters.
3.Nucleic Acid Extraction Experiments: DNA extraction from blood and tissue, RNA extraction, preservation, etc. Nucleic acids are key materials for conducting SNP research, sequencing, and gene upregulation or downregulation studies. The Central Laboratory has the conditions for  nucleic acid extraction and preservation.
4.SDS-PAGE Experiments: SDS-Polyacrylamide Gel Electrophoresis experiments for analyzing proteins expressed in samples. A Western Blot immuno-electrophoresis imaging system has been purchased, enabling the completion of Western Blot experiments.
5.PCR Experiments: Conventional PCR experiments, fluorescent quantitative PCR experiments, which can be used for the amplification, analysis, and identification of DNA or RNA extracted from samples.
6.Biochemical Index Detection: Can perform detection of biochemical indicators such as Superoxide Dismutase (SOD), Catalase (CAT), Peroxidase (POD), and protein content in serum, plasma, and tissue samples.
7.ELISA Experiments: Can perform various ELISA assays on serum or plasma samples. The Central Laboratory can conduct measurements of cytokines and antibodies in samples.